Product Name :
MAPK13 polyclonal antibody Background :
Lipopolysaccharide has been shown to induce tyrosine phosphorylation of a unique protein, which is designated p38. p38 is a member of the MAP kinase family with features most closely resembling those of the Saccharo-myces cerevisiae protein Hog1. p38 and Hog1 share a TGY phosphorylation sequence, whereas most other MAP kinase family proteins have a TEY sequence. A related protein, p38b, has been shown to phosphorylate ATF-2 at a 20-fold higher rate than p38, suggesting distinct substrate preferences. Stress activated protein kinase-4, or SAPK4, also designated p38d, is a related protein that is phosphorylated by MKK6 in response to cytokines and cellular stresses. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MAPK13 polyclonal antibody detects endogenous levels of MAPK13 protein. Immunogen :
A synthetic peptide corresponding to residues in Human MAPK13 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of MAPK13 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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