Product Name :
EPO polyclonal antibody Background :
Erythropoietin, or Epo, is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. In addition, Epo has also been shown to play a role in primitive embryonic erythropoiesis. Epo is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. Circulating Epo is a 165 amino acid glycoprotein. The Epo receptor, EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Overexpression of Epo is associated with several pathophysiological conditions, such as polycythemias vera, which is caused by the Epo-independent growth of erythrocytic progenitors from abnormal stem cells. A deficiency in Epo expression has been associated with afflictions such as anemia of chronic disease (ACD), frequently found in rheumatoid arthritis (RA) patients. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
EPO polyclonal antibody detects endogenous levels of EPO protein. Immunogen :
A synthetic peptide corresponding to residues in Human EPO. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of EPO polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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