Product Name :
GPR182 polyclonal antibody Background :
Adrenomedullin (ADM) is a hypotensive peptide that belongs to a peptide superfamily, which includes the calcitonin gene-related peptide (CGRP) and amylin . ADM was originally identified in the adrenal medulla, where it is highly expressed . It is also produced by most contractile cells and is upregulated during sepsis and ischemia. Three distinct receptors have the ability to bind ADM and are designated ADM receptor (also designated L1), RDC-1 and the Calcitonin Receptor-Like Receptor (CRLR) . The CRLR associates with receptor activity-modifying proteins (RAMPs), which determine the specificity of CRLR binding . Co-expression with RAMP1 results in CRLR binding to CGRP, whereas association with RAMP2 or 3 results in ADM binding . The ADM receptor, a seven transmembrane G protein-coupled receptor, specifically binds ADM and is highly expressed in heart, brain, skeletal muscle, the immune system, adrenal gland and liver. The ADM receptor mediates the signals produced by ADM through G proteins, which activate adenylate cyclase . Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GPR182 polyclonal antibody detects endogenous levels of GPR182 protein. Immunogen :
A synthetic peptide corresponding to residues in Human GPR182. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of GPR182 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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