Product Name :
RAD1 polyclonal antibody Background :
DNA damage or incomplete replication of DNA results in inhibition of cell cycle progression at the G1-S or G2-M checkpoints by conserved regulatory mechanisms. Rad17 is involved in regulation of cell cycle arrest at the G1 checkpoint, whereas Chk1, Rad1, Rad9 and Hus1 are involved in regulation of cell cycle arrest at the G2 checkpoint. Over- expression of Rad17 results in p53 activation and an accumulation of cells in G1 phase. Chk1 functions as an essential component in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage, thus inhibiting mitosis. Hus1 and Rad9 exhibit conserved function in fission yeast and higher eukaryotes. Hus1 has been shown to be phosphorylated in response to DNA damage, a process which requires rad checkpoint genes. Rad9 is thought to be a candidate tumor sup-pressor gene because it is localized to a region of human chromosome 11 containing a number of tumor suppressor loci. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RAD1 polyclonal antibody detects endogenous levels of RAD1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RAD1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RAD1 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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