RRAS2 polyclonal antibody 
Datasheet
COA
MSDS
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Product Name :
RRAS2 polyclonal antibody Background :
H-, K- and N-Ras represent the prototype members of a family of small G proteins that are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at either position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound, rather than the inactive GDP-bound, state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 and 87 (analogous to position 12 and 61 in H-Ras) mutants of R-Ras have been shown to be capable of activating oncogenic function. An additional member of the Ras oncogene family, designated TC 21 (or R-Ras-2) is most closely related to R-Ras. While wild type TC 21 does not exhibit transforming potential in vitro, mutant forms of TC 21 that possess amino acid substitutions analogous to those that activate Ras oncogenic potential, exhibit potent transforming activities comparable to the activity characteristic of the known oncogenic Ras proteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RRAS2 polyclonal antibody detects endogenous levels of RRAS2 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RRAS2. Conjugate :
Unconjugated Modification :
Unmodification
RRAS2 polyclonal antibody Background :
H-, K- and N-Ras represent the prototype members of a family of small G proteins that are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at either position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound, rather than the inactive GDP-bound, state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 and 87 (analogous to position 12 and 61 in H-Ras) mutants of R-Ras have been shown to be capable of activating oncogenic function. An additional member of the Ras oncogene family, designated TC 21 (or R-Ras-2) is most closely related to R-Ras. While wild type TC 21 does not exhibit transforming potential in vitro, mutant forms of TC 21 that possess amino acid substitutions analogous to those that activate Ras oncogenic potential, exhibit potent transforming activities comparable to the activity characteristic of the known oncogenic Ras proteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RRAS2 polyclonal antibody detects endogenous levels of RRAS2 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RRAS2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RRAS2 polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:sp2/0 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.