Product Name :
POLR2I polyclonal antibody Background :
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. POLR2I (polymerase (RNA) II (DNA directed) polypeptide I), also known as RPB9 or hRPB14.5, is a 125 amino acid nuclear protein belonging to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. Component of RNA polymerase II, POLR2I catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2I is part of the upper jaw surrounding the central large cleft and is thought to grab the incoming DNA template. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
POLR2I polyclonal antibody detects endogenous levels of POLR2I protein. Immunogen :
A synthetic peptide corresponding to residues in Human POLR2I. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of POLR2I pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(30ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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