CHERP polyclonal antibody 
Datasheet
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Product Name :
CHERP polyclonal antibody Background :
The regulation of the intracellular concentration of calcium is important for proper maintenance of voltage-gated ion channels which control muscle and nerve function. Calcium homeostasis is regulated by a variety of proteins. CHERP (calcium homeostasis endoplasmic reticulum protein), also known as SRA1, DAN16 or SCAF6, is a 916 amino acid protein that localizes to the cytoplasm and the endoplasmic reticulum (ER). Expressed in pancreas, brain, lung, placenta, liver, kidney, heart and skeletal muscle, CHERP is involved in maintaining calcium homeostasis and plays a role in cell growth and proliferation. CHERP contains one G-patch domain, one RPR domain and one SURP motif and is expressed as two isoforms due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHERP polyclonal antibody detects endogenous levels of CHERP protein. Immunogen :
A synthetic peptide corresponding to residues in Human CHERP. Conjugate :
Unconjugated Modification :
Unmodification
CHERP polyclonal antibody Background :
The regulation of the intracellular concentration of calcium is important for proper maintenance of voltage-gated ion channels which control muscle and nerve function. Calcium homeostasis is regulated by a variety of proteins. CHERP (calcium homeostasis endoplasmic reticulum protein), also known as SRA1, DAN16 or SCAF6, is a 916 amino acid protein that localizes to the cytoplasm and the endoplasmic reticulum (ER). Expressed in pancreas, brain, lung, placenta, liver, kidney, heart and skeletal muscle, CHERP is involved in maintaining calcium homeostasis and plays a role in cell growth and proliferation. CHERP contains one G-patch domain, one RPR domain and one SURP motif and is expressed as two isoforms due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHERP polyclonal antibody detects endogenous levels of CHERP protein. Immunogen :
A synthetic peptide corresponding to residues in Human CHERP. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CHERP polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.