Product Name :
WIG1 polyclonal antibody Background :
p53 is a DNA-binding protein that is involved in a variety of processes, including tumor suppression and apoptosis, DNA replication and repair, and cell cycle regulation. Normal cells and tissues express a low level of p53 under most circumstances, but p53 expression is induced by DNA damage and cellular stress. Wig-1 (wild type p53-induced gene 1) is a zinc finger protein that contains a putative nuclear localization signal (NLS) and is induced by p53. Wig-1 expression is increased by whole body gamma irradiation in these tissues as well as in spleen and lung. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
WIG1 polyclonal antibody detects endogenous levels of WIG1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human WIG1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of WIG1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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