Product Name :
CIB1 polyclonal antibody Background :
Platelets regulate the function of Integrin α2b/β3 (GPIIb/IIIa), the platelet Fibrinogen receptor, which is involved in the binding of proteins to integrin cytoplasmic domains. A novel protein, CIB, for calcium- and integrin-binding protein (also designated as Kip for kinase interacting protein, SIP2-28 and DNA-PKcs interacting protein), binds specifically at the cytoplasmic domain of α2b by a number of positively charged residues in its binding site. Binding of CIB to the α2b is affected by fluctuations in the intracellular calcium concentration. In aggregated platelets, endogenous CIB and α2b/β3 translocate to the Triton X-100-insoluble cytoskeleton, demonstrating that the cellular localization of CIB is regulated. CIB also binds to DNA-PKcs, which is a nu-clear protein serine/threonine kinase that plays a role in the DNA repair and recombination process of lymphoid development. Fnk also binds to the CIB, suggesting that CIB may be a regulatory subunit of polo-like kinases. CIB shows significant homology to calcineurin B and calmodulin, and its mRNA levels are ubiquitously expressed in various human tissues. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CIB1 polyclonal antibody detects endogenous levels of CIB1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human CIB1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CIB1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:H9C2 whole cell lysate Lane3:Rat brain tissue lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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