FOXN1 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
FOXN1 polyclonal antibody Background :
The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXN1, also designated Transcription factor winged-nude (WHN), is required for keratinocyte growth, as well as differentiation of epithelial progenitor cells in the thymic primordium into subcapsular, cortical, and medullary epithelial cells of the mature thymus. Mutations in the FOXN1 gene are responsible for nude, immune-deficient mice and rats. These nude mice are useful as hosts for xenografts in cancer research. The promoters for FOXN1 are active in the skin and thymus reflecting the critical role FOXN1 plays in the proper development of these tissues. Secreted Wnt glycoproteins appear to regulate FOXN1 transcription in the thymus. FOXN1 is expressed in the embryonic thymus after the common primordium is formed, beginning at E11.25. FOXN1 is also expressed at very low levels in normal human kidney and thyroid gland. In human, it is also expressed in the differentiating cells of the hair follicle precortex, the innermost layer of the outer root sheath, and the thymus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FOXN1 polyclonal antibody detects endogenous levels of FOXN1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human FOXN1. Conjugate :
Unconjugated Modification :
Unmodification
FOXN1 polyclonal antibody Background :
The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXN1, also designated Transcription factor winged-nude (WHN), is required for keratinocyte growth, as well as differentiation of epithelial progenitor cells in the thymic primordium into subcapsular, cortical, and medullary epithelial cells of the mature thymus. Mutations in the FOXN1 gene are responsible for nude, immune-deficient mice and rats. These nude mice are useful as hosts for xenografts in cancer research. The promoters for FOXN1 are active in the skin and thymus reflecting the critical role FOXN1 plays in the proper development of these tissues. Secreted Wnt glycoproteins appear to regulate FOXN1 transcription in the thymus. FOXN1 is expressed in the embryonic thymus after the common primordium is formed, beginning at E11.25. FOXN1 is also expressed at very low levels in normal human kidney and thyroid gland. In human, it is also expressed in the differentiating cells of the hair follicle precortex, the innermost layer of the outer root sheath, and the thymus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FOXN1 polyclonal antibody detects endogenous levels of FOXN1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human FOXN1. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of FOXN1 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.