ZNF460 polyclonal antibody 
Datasheet
COA
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Product Name :
ZNF460 polyclonal antibody Background :
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF460 (zinc finger protein 460), also known as HZF8 or ZNF272, is a 562 amino acid nuclear protein that is ubiquitously expressed at low levels and expressed at high levels in pancreas and liver. Belonging to the Krueppel C2 H2-type zinc-finger protein family, ZNF460 contains 11 C2 H2-type zinc fingers and a KRAB domain. Like other zinc-fingers, ZNF460 is also suggested to be involved in transcriptional regulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ZNF460 polyclonal antibody detects endogenous levels of ZNF460 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 506-551 of Human ZNF460. Conjugate :
Unconjugated Modification :
Unmodification
ZNF460 polyclonal antibody Background :
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF460 (zinc finger protein 460), also known as HZF8 or ZNF272, is a 562 amino acid nuclear protein that is ubiquitously expressed at low levels and expressed at high levels in pancreas and liver. Belonging to the Krueppel C2 H2-type zinc-finger protein family, ZNF460 contains 11 C2 H2-type zinc fingers and a KRAB domain. Like other zinc-fingers, ZNF460 is also suggested to be involved in transcriptional regulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ZNF460 polyclonal antibody detects endogenous levels of ZNF460 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 506-551 of Human ZNF460. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ZNF460 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.