Product Name :
TNFα-IP8 polyclonal antibody Background :
TNFα-IP 8 (tumor necrosis factor, α-induced protein 8), also known as NFκB-inducible DED-containing protein (NDED), SCC-S2 or TNF-induced protein GG2-1, is a 198 amino acid cytoplasmic protein induced by NFκB and TNF. The induction of TNFα-IP 8 by TNF is dependent on the activation of NFκB. TNFα-IP 8 negatively mediates apoptosis and may also play a role in tumor progression. TNFα-IP 8 specifically inhibits caspase-8 activity, which results in the inhibition of BID cleavage and caspase-3 activation during TNF-mediated apoptosis. TNFα-IP 8 is expressed at high levels in thymus, bone marrow, lymph node, spleen, thyroid, placenta and various tumor tissues, as well as fetal lung, liver and kidney. TNFα-IP 8 is present as three isoforms produced by alternative splicing. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TNFα-IP8 polyclonal antibody detects endogenous levels of TNFα-IP8 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 71-116 of Human TNFα-IP83. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TNFα-IP8 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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