SLU7 polyclonal antibody 
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Product Name :
SLU7 polyclonal antibody Background :
In order to produce correctly spliced messenger RNA, two catalytic splicing steps are required. After catalytic step I, a major remodeling of the spliceosome occurs to establish the active site for step II. During the second step of mRNA splicing, exon 1 attacks an adenine-guanine (AG) dinucleotide at the 3' splice site. SLU7, the human homolog of the yeast step II splice factor Slu7, is required for selection of the correct AG. Human SLU7 associates with the spliceosome late in the splicing pathway prior to recognition of the 3' splice site for step II. SLU7 depletion in HeLa nuclear extract reveals that SLU7 is required to hold exon 1 tightly within the spliceosome for attack on a prespecified AG. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SLU7 polyclonal antibody detects endogenous levels of SLU7 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 154-199 of Human SLU7. Conjugate :
Unconjugated Modification :
Unmodification
SLU7 polyclonal antibody Background :
In order to produce correctly spliced messenger RNA, two catalytic splicing steps are required. After catalytic step I, a major remodeling of the spliceosome occurs to establish the active site for step II. During the second step of mRNA splicing, exon 1 attacks an adenine-guanine (AG) dinucleotide at the 3' splice site. SLU7, the human homolog of the yeast step II splice factor Slu7, is required for selection of the correct AG. Human SLU7 associates with the spliceosome late in the splicing pathway prior to recognition of the 3' splice site for step II. SLU7 depletion in HeLa nuclear extract reveals that SLU7 is required to hold exon 1 tightly within the spliceosome for attack on a prespecified AG. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SLU7 polyclonal antibody detects endogenous levels of SLU7 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 154-199 of Human SLU7. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of SLU7 (F144) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.