PEBP1 polyclonal antibody 
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MSDS
SHIP
Product Name :
PEBP1 polyclonal antibody Background :
Raf kinase inhibitor protein (RKIP) is a cytosolic protein that was initially characterized as a phosphatidylethanolamine-binding protein (PBP) expressed in brain tissue and secreted from testis fluid. In addition, RKIP was identified by yeast two-hybrid screening of human T cell libraries directed at identifying proteins that associate with the BXB kinase domain of the serine/threonine kinase, Raf-1. Subsequent in vitro and in vivo studies indicate that RKIP binds to both the active and inactive forms of Raf-1 and thereby regulates the signaling cascade of the MAP kinase pathway. The specific association of RKIP with kinase-active Raf-1 competitively inhibits the binding and activation of the Raf-1 substrate MEK. RKIP, in turn, affects downstream MAP kinase signaling by decreasing the activation of MEK effector proteins, including ERK1 and ERK2, and the subsequent induction of AP-1 mediated transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PEBP1 polyclonal antibody detects endogenous levels of PEBP1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 142-187 of Human PEBP1. Conjugate :
Unconjugated Modification :
Unmodification
PEBP1 polyclonal antibody Background :
Raf kinase inhibitor protein (RKIP) is a cytosolic protein that was initially characterized as a phosphatidylethanolamine-binding protein (PBP) expressed in brain tissue and secreted from testis fluid. In addition, RKIP was identified by yeast two-hybrid screening of human T cell libraries directed at identifying proteins that associate with the BXB kinase domain of the serine/threonine kinase, Raf-1. Subsequent in vitro and in vivo studies indicate that RKIP binds to both the active and inactive forms of Raf-1 and thereby regulates the signaling cascade of the MAP kinase pathway. The specific association of RKIP with kinase-active Raf-1 competitively inhibits the binding and activation of the Raf-1 substrate MEK. RKIP, in turn, affects downstream MAP kinase signaling by decreasing the activation of MEK effector proteins, including ERK1 and ERK2, and the subsequent induction of AP-1 mediated transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PEBP1 polyclonal antibody detects endogenous levels of PEBP1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 142-187 of Human PEBP1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RKIP (L138) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:The Testis tissue lysate of Rat(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.