RPS2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RPS2 polyclonal antibody Background :
Bacterial pathogens deliver type III effector proteins into the plant cell during infection. Plants express disease resistance (R) proteins that respond specifically to a particular type III effector by activating immune responses. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction. RPS2 (resistance to Pseudomonas syringae protein 2) is a 909 amino acid plasma membrane protein with leucine-rich repeat, leucine zipper and P loop domains that confers resistance to Pseudomonas syringae infection by interacting with the avirulence gene AvrRpt2. Belonging to the disease resistance NB-LRR family, RPS2 directly associates with RIN4, which triggers plant resistance when RIN4 is degraded by AvrRpt2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RPS2 polyclonal antibody detects endogenous levels of RPS2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 248-293 of Human RPS2. Conjugate :
Unconjugated Modification :
Unmodification
RPS2 polyclonal antibody Background :
Bacterial pathogens deliver type III effector proteins into the plant cell during infection. Plants express disease resistance (R) proteins that respond specifically to a particular type III effector by activating immune responses. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction. RPS2 (resistance to Pseudomonas syringae protein 2) is a 909 amino acid plasma membrane protein with leucine-rich repeat, leucine zipper and P loop domains that confers resistance to Pseudomonas syringae infection by interacting with the avirulence gene AvrRpt2. Belonging to the disease resistance NB-LRR family, RPS2 directly associates with RIN4, which triggers plant resistance when RIN4 is degraded by AvrRpt2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RPS2 polyclonal antibody detects endogenous levels of RPS2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 248-293 of Human RPS2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RPS2 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.