RPL3 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RPL3 polyclonal antibody Background :
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L3, also known as RPL3 or TARBP-B, is a 403 amino acid protein that localizes to the cytoplasm and belongs to the L3P family of ribosomal proteins. Expressed as multiple alternatively spliced isoforms, Ribosomal Protein L3 is able to bind to HIV-1 mRNA, possibly activating HIV-1 protein translation. Like most ribosomal proteins, Ribosomal Protein L3 exists as multiple processed pseudogenes that are scattered throughout the genome. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RPL3 polyclonal antibody detects endogenous levels of RPL3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 262-307 of Human RPL3. Conjugate :
Unconjugated Modification :
Unmodification
RPL3 polyclonal antibody Background :
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L3, also known as RPL3 or TARBP-B, is a 403 amino acid protein that localizes to the cytoplasm and belongs to the L3P family of ribosomal proteins. Expressed as multiple alternatively spliced isoforms, Ribosomal Protein L3 is able to bind to HIV-1 mRNA, possibly activating HIV-1 protein translation. Like most ribosomal proteins, Ribosomal Protein L3 exists as multiple processed pseudogenes that are scattered throughout the genome. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RPL3 polyclonal antibody detects endogenous levels of RPL3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 262-307 of Human RPL3. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RPL3 polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:SK-OVCAR3 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:EC9706 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.