RASSF2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RASSF2 polyclonal antibody Background :
Ras is a small GTP-binding protein involved in many cellular processes, including proliferation, differentiation and apoptosis. Ras transmits signals of cell surface receptors by binding to a variety of effector molecules. In addition to the well-characterized effectors Raf and PI-3 kinase, Ras also interacts with a group of homologous, noncatalytic proteins composed of RASSF1, RASSF2, RASSF3, AD037 and Nore1. RASSF1 is a potential tumor suppressor gene that plays an important role in tumor pathogenesis. Nore1 binds to Ras in response to EGF or serum stimulation, but its function has yet to be determined. RASSF2 is a nuclear protein containing a Ras-associating domain and a SARAH domain. RASSF2 isoform A is inactivated in colorectal cancer cells by CpG island promoter hypermethylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RASSF2 polyclonal antibody detects endogenous levels of RASSF2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 152-197 of Human RASSF2. Conjugate :
Unconjugated Modification :
Unmodification
RASSF2 polyclonal antibody Background :
Ras is a small GTP-binding protein involved in many cellular processes, including proliferation, differentiation and apoptosis. Ras transmits signals of cell surface receptors by binding to a variety of effector molecules. In addition to the well-characterized effectors Raf and PI-3 kinase, Ras also interacts with a group of homologous, noncatalytic proteins composed of RASSF1, RASSF2, RASSF3, AD037 and Nore1. RASSF1 is a potential tumor suppressor gene that plays an important role in tumor pathogenesis. Nore1 binds to Ras in response to EGF or serum stimulation, but its function has yet to be determined. RASSF2 is a nuclear protein containing a Ras-associating domain and a SARAH domain. RASSF2 isoform A is inactivated in colorectal cancer cells by CpG island promoter hypermethylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RASSF2 polyclonal antibody detects endogenous levels of RASSF2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 152-197 of Human RASSF2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RASSF2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.