PHKG1 polyclonal antibody 
Datasheet
COA
MSDS
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Product Name :
PHKG1 polyclonal antibody Background :
PHKG1 (phosphorylase kinase subunit γ1), is a subunit of phosphorylase kinase (PHK) that belongs to the Ser/Thr protein kinase family. PHK is a hexadecameric protein composed of four α chains, four β chains, four γ chains and four δ chains. The γ chains are catalytic chains, the α and β chains are regulatory chains and the δ chains are calmodulins. PHKG1 contains two calmodulin-binding domains and one protein kinase domain. As the catalytic chain of PHK, PHKG1 is responsible for catalyzing the phosphorylation and activation of glycogen phosphorylase and therefore it plays an important role in the glycogenolytic pathway. Mutations in the gene encoding PHKG1 can lead to PHK deficiency and result in glycogen storage disease type 9C (GSD9C), also known as autosomal liver glycogenosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PHKG1 polyclonal antibody detects endogenous levels of PHKG1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 293-338 of Human PHKG1. Conjugate :
Unconjugated Modification :
Unmodification
PHKG1 polyclonal antibody Background :
PHKG1 (phosphorylase kinase subunit γ1), is a subunit of phosphorylase kinase (PHK) that belongs to the Ser/Thr protein kinase family. PHK is a hexadecameric protein composed of four α chains, four β chains, four γ chains and four δ chains. The γ chains are catalytic chains, the α and β chains are regulatory chains and the δ chains are calmodulins. PHKG1 contains two calmodulin-binding domains and one protein kinase domain. As the catalytic chain of PHK, PHKG1 is responsible for catalyzing the phosphorylation and activation of glycogen phosphorylase and therefore it plays an important role in the glycogenolytic pathway. Mutations in the gene encoding PHKG1 can lead to PHK deficiency and result in glycogen storage disease type 9C (GSD9C), also known as autosomal liver glycogenosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PHKG1 polyclonal antibody detects endogenous levels of PHKG1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 293-338 of Human PHKG1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PHKG1 polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat brain tissue lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.