Product Name :
Peroxin-11B polyclonal antibody Background :
Peroxisomal membrane protein 11B is a protein that in humans is encoded by the PEX11B gene. Involved in peroxisomal proliferation. May regulate peroxisomes division by recruiting the dynamin-related GTPase DNM1L to the peroxisomal membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Peroxin-11B polyclonal antibody detects endogenous levels of Peroxin-11B protein. Immunogen :
Synthetic peptide, corresponding to amino acids 132-177 of Human Peroxin-11B. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Peroxin-11B polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate
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  Immunohistochemistry (IHC) analyzes of Peroxin-11B pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing Peroxisome membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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