PDE4C polyclonal antibody 
Datasheet
COA
MSDS
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Product Name :
PDE4C polyclonal antibody Background :
Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4C gene maps to chromosome 19p13.1. The splice variants include isoforms PDE4C1-7. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PDE4C polyclonal antibody detects endogenous levels of PDE4C protein. Immunogen :
Synthetic peptide, corresponding to amino acids 333-378 of Human PDE4C. Conjugate :
Unconjugated Modification :
Unmodification
PDE4C polyclonal antibody Background :
Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4C gene maps to chromosome 19p13.1. The splice variants include isoforms PDE4C1-7. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PDE4C polyclonal antibody detects endogenous levels of PDE4C protein. Immunogen :
Synthetic peptide, corresponding to amino acids 333-378 of Human PDE4C. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PDE4C polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Mouse liver tissue lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.