ARHGEF5 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ARHGEF5 polyclonal antibody Background :
Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors. ARHGEF5 (Rho guanine nucleotide exchange factor (GEF) 5), also known as P60 or transforming immortalized mammary oncogene (TIM), is a 1597 amino acid cytoplasmic protein. ARHGEF5 is believed to form a complex with G proteins and be involved in the initiation of Rho-dependent signals. It has also been suggested that ARHGEF5 may be involved in the organization of the cytoskeleton. ARHGEF5 contains one PH domain, one DH domain and one SH3 domain. Existing as two isoforms produced by alternative splicing, ARHGEF5 is mainly expressed in pancreas, liver, kidney, placenta and lung. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF5 polyclonal antibody detects endogenous levels of ARHGEF5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1310-1355 of Human ARHGEF5. Conjugate :
Unconjugated Modification :
Unmodification
ARHGEF5 polyclonal antibody Background :
Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors. ARHGEF5 (Rho guanine nucleotide exchange factor (GEF) 5), also known as P60 or transforming immortalized mammary oncogene (TIM), is a 1597 amino acid cytoplasmic protein. ARHGEF5 is believed to form a complex with G proteins and be involved in the initiation of Rho-dependent signals. It has also been suggested that ARHGEF5 may be involved in the organization of the cytoskeleton. ARHGEF5 contains one PH domain, one DH domain and one SH3 domain. Existing as two isoforms produced by alternative splicing, ARHGEF5 is mainly expressed in pancreas, liver, kidney, placenta and lung. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF5 polyclonal antibody detects endogenous levels of ARHGEF5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1310-1355 of Human ARHGEF5. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ARHGEF5 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.