NPAS1 polyclonal antibody 
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Product Name :
NPAS1 polyclonal antibody Background :
Members of the bHLH-PAS family are transcription factors that contain a basic helix-loop-helix (bHLH) DNA-recognition motif which is located N-terminal to a PAS domain comprised of two imperfect direct repeats. Human NPAS1 is a deduced 590-amino acid protein which shares 86% sequence homology with mouse Npas1. In order for NPAS1 to bind DNA efficiently, it must form a dimer with another bHLH protein. NPAS1 interacts with ARNT (aryl hydrocarbon receptor nuclear translocator), and shows predominant expression in brain tissue. NPAS1 is also implicated in the control of regulatory pathways relevant to schizophrenia and to psychotic illness, and may play a role in late central nervous system development by modulating EPO expression in response to cellular oxygen levels. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NPAS1 polyclonal antibody detects endogenous levels of NPAS1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 486-531 of Human NPAS1. Conjugate :
Unconjugated Modification :
Unmodification
NPAS1 polyclonal antibody Background :
Members of the bHLH-PAS family are transcription factors that contain a basic helix-loop-helix (bHLH) DNA-recognition motif which is located N-terminal to a PAS domain comprised of two imperfect direct repeats. Human NPAS1 is a deduced 590-amino acid protein which shares 86% sequence homology with mouse Npas1. In order for NPAS1 to bind DNA efficiently, it must form a dimer with another bHLH protein. NPAS1 interacts with ARNT (aryl hydrocarbon receptor nuclear translocator), and shows predominant expression in brain tissue. NPAS1 is also implicated in the control of regulatory pathways relevant to schizophrenia and to psychotic illness, and may play a role in late central nervous system development by modulating EPO expression in response to cellular oxygen levels. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NPAS1 polyclonal antibody detects endogenous levels of NPAS1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 486-531 of Human NPAS1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NPAS1 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat kidney tissue lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.