MRP-S12 polyclonal antibody 
Datasheet
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MSDS
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Product Name :
MRP-S12 polyclonal antibody Background :
Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRP-S12 (mitochondrial ribosomal protein S12) is a 138 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. In response to mitochondrial stress, bidirectional MRP-S12 promoter activity is strongly stimulated, an event that happens to correlate with mitochondrial reactive oxidative species (ROS) production. Due to its specific location on human chromosome 19, the gene encoding MRP-S12 may be a candidate gene for susceptibility to aminoglycoside ototoxicity and for the autosomal dominant deafness gene DFNA4. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MRP-S12 polyclonal antibody detects endogenous levels of MRP-S12 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 69-114 of Human MRP-S12. Conjugate :
Unconjugated Modification :
Unmodification
MRP-S12 polyclonal antibody Background :
Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRP-S12 (mitochondrial ribosomal protein S12) is a 138 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. In response to mitochondrial stress, bidirectional MRP-S12 promoter activity is strongly stimulated, an event that happens to correlate with mitochondrial reactive oxidative species (ROS) production. Due to its specific location on human chromosome 19, the gene encoding MRP-S12 may be a candidate gene for susceptibility to aminoglycoside ototoxicity and for the autosomal dominant deafness gene DFNA4. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MRP-S12 polyclonal antibody detects endogenous levels of MRP-S12 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 69-114 of Human MRP-S12. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of MRP-S12 (K59) pAb at 1:1000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.