CYP2E1 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CYP2E1 polyclonal antibody Background :
Cytochrome P450 2E1 (CYP2E1) localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous levels of substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. CYP2E1 plays an important role in alcohol metabolism and participates in the metabolic activation of various carcinogens. Chronic ethanol consumption results in the induction of hepatic CYP2E1 in humans, which may play an important role in the pathogenesis of alcoholic liver disease. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP2E1 polyclonal antibody detects endogenous levels of CYP2E1 protein. Immunogen :
Synthetic peptide, corresponding to the 411-456 of Human CYP2E1. Conjugate :
Unconjugated Modification :
Unmodification
CYP2E1 polyclonal antibody Background :
Cytochrome P450 2E1 (CYP2E1) localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous levels of substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. CYP2E1 plays an important role in alcohol metabolism and participates in the metabolic activation of various carcinogens. Chronic ethanol consumption results in the induction of hepatic CYP2E1 in humans, which may play an important role in the pathogenesis of alcoholic liver disease. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP2E1 polyclonal antibody detects endogenous levels of CYP2E1 protein. Immunogen :
Synthetic peptide, corresponding to the 411-456 of Human CYP2E1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CYP2E1 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.