CYP2A7 polyclonal antibody 
Datasheet
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MSDS
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Product Name :
CYP2A7 polyclonal antibody Background :
The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze reactions involved in both drug metabolism and in the synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A and CYP2A, based on their sequence similarities. CYP2A7 (cytochrome P450 2A7), also known as CPA7, CPAD, CYPIIA7 or P450-IIA4, is a 494 amino acid peripheral membrane protein that belongs to the CYP2A subfamily of cytochrome P450 proteins. Localized to the endoplasmic reticulum, CYP2A7 can be induced to high levels in liver in response to foreign compounds, such as carcinogens, pesticides and drugs. CYP2A7 functions as a hydroxylase that uses a heme group to catalyze the oxidation of target flavoproteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP2A7 polyclonal antibody detects endogenous levels of CYP2A7 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 435-480 of Human CYP2A7. Conjugate :
Unconjugated Modification :
Unmodification
CYP2A7 polyclonal antibody Background :
The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze reactions involved in both drug metabolism and in the synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A and CYP2A, based on their sequence similarities. CYP2A7 (cytochrome P450 2A7), also known as CPA7, CPAD, CYPIIA7 or P450-IIA4, is a 494 amino acid peripheral membrane protein that belongs to the CYP2A subfamily of cytochrome P450 proteins. Localized to the endoplasmic reticulum, CYP2A7 can be induced to high levels in liver in response to foreign compounds, such as carcinogens, pesticides and drugs. CYP2A7 functions as a hydroxylase that uses a heme group to catalyze the oxidation of target flavoproteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP2A7 polyclonal antibody detects endogenous levels of CYP2A7 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 435-480 of Human CYP2A7. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CYP2A7 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.