COX5A polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
COX5A polyclonal antibody Background :
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5a, one of two subunits of the COX5 protein, is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. When oxygen levels within the cell are high, transcription of COX5a, the aerobic subunit of the COX5 protein, is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5a transcription decreases as the cell works to conserve oxygen by slowing the creation of water. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COX5A polyclonal antibody detects endogenous levels of COX5A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 53-98 of Human COX5A. Conjugate :
Unconjugated Modification :
Unmodification
COX5A polyclonal antibody Background :
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5a, one of two subunits of the COX5 protein, is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. When oxygen levels within the cell are high, transcription of COX5a, the aerobic subunit of the COX5 protein, is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5a transcription decreases as the cell works to conserve oxygen by slowing the creation of water. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COX5A polyclonal antibody detects endogenous levels of COX5A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 53-98 of Human COX5A. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of COX5A polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate -
Immunohistochemistry (IHC) analyzes of COX5A pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing Mitochondrion inner membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats
PMCID: Pubmed No.:26058943
NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats
PMCID: Pubmed No.:26058943
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.