ARHGEF10 polyclonal antibody 
Datasheet
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Product Name :
ARHGEF10 polyclonal antibody Background :
ARHGEF10 (rho guanine nucleotide exchange factor 10), also known as GEF10 or KIAA0294, is a 1,369 amino acid protein that contains one DBL-homology domain and is thought to play a role in myelination of peripheral nerves, specifically during development. ARHGEF10 is expressed as four alternatively spliced isoforms that are present at low levels in ovary, lung, testis and kidney, with considerably higher expression in the central and peripheral nervous systems. Defects in the gene encoding ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV), an autosomal dominant disorders that is characterized by a reduction in nerve velocities resulting from lack of proper myelination. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF10 polyclonal antibody detects endogenous levels of ARHGEF10 protein. Immunogen :
A synthetic peptide corresponding to residues in Human ARHGEF10. Conjugate :
Unconjugated Modification :
Unmodification
ARHGEF10 polyclonal antibody Background :
ARHGEF10 (rho guanine nucleotide exchange factor 10), also known as GEF10 or KIAA0294, is a 1,369 amino acid protein that contains one DBL-homology domain and is thought to play a role in myelination of peripheral nerves, specifically during development. ARHGEF10 is expressed as four alternatively spliced isoforms that are present at low levels in ovary, lung, testis and kidney, with considerably higher expression in the central and peripheral nervous systems. Defects in the gene encoding ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV), an autosomal dominant disorders that is characterized by a reduction in nerve velocities resulting from lack of proper myelination. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF10 polyclonal antibody detects endogenous levels of ARHGEF10 protein. Immunogen :
A synthetic peptide corresponding to residues in Human ARHGEF10. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ARHGEF10 (R370) pAb at 1:1000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:3T3-L1 whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.