Product Name :
ERK1/2 (phospho-T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at threonine 202 and tyrosine 204, and ERK2 protein when phosphorylated at threonine 185 and tyrosine 187. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204. Conjugate :
Unconjugated Modification :
Phosphorylation
ERK1/2 (phospho-T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at threonine 202 and tyrosine 204, and ERK2 protein when phosphorylated at threonine 185 and tyrosine 187. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-ERK1/2 (T202/Y204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate treated with UV(4h) Lane2:Raw264.7 whole cell lysate treated with UV(4h) Lane3:PC12 whole cell lysate treated with UV(4h)
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Immunohistochemistry (IHC) analyzes of p-ERK1/2 (T202/Y204) pAb in paraffin-embedded human breast carcinoma tissue.
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Regulator of G Protein Signalling 5 protects against atherosclerosis in apolipoprotein E-deficient mice.
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Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways
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NF-κB, ERK, p38 MAPK and JNK contribute to the initiation and/or maintenance of mechanical allodynia induced by tumor necrosis factor-alpha in the red nucleus
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Cardioprotection of Vitexin on Myocardial Ischemia/Reperfusion Injury in Rat via Regulating Inflammatory Cytokines and MAPK Pathway
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ERK MAP kinase activation in spinal cord regulates phosphorylation of Cdk5 at serine 159 and contributes to peripheral inflammation induced pain/hypersensitivity.
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GPER-mediated proliferation and estradiol production in breast cancer-associated fibroblasts.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.