Product Name :
PDLIM5 (phospho-Y251) polyclonal antibody Background :
PDLIM5 (PDZ and LIM domain 5), also known as L9, ENH (Enigma homolog),LIM or ENH1, is a member of the Enigma family of proteins. Proteins belonging to this family contain an N-terminal PDZ (post-synaptic density-95/discs large/zone occludens-1) domain and one to three C-terminal LIM domains that typically associate with various isoforms of PKC (protein kinase C). Expressed in a wide variety of tissues, PDLIM5 contains three LIM zinc-binding domains and one PDZ domain. In the brain, PDLIM5 colocalizes with synaptic vesicles of neurotransmitters and regulates neuronal calcium signaling through an interaction with PKC ε and N-type Ca++ CP α1B (N-type calcium channel α1B subunit). Expression of PDLIM5 is often increased in the brain of patients with schizophrenia, major depression and bipolar disorder. This suggests that PDLIM5 may contribute to the genetic susceptibility of such conditions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PDLIM5 (Y251) polyclonal antibody detects endogenous levels of PDLIM5 protein only when phosphorylated at Tyr251. Immunogen :
Synthetic phosphopeptide derived from human PDLIM5 around the phosphorylation site of Tyrosine 251. Conjugate :
Unconjugated Modification :
Phosphorylation

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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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