eIF2α (phospho-S51) polyclonal antibody 
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Product Name :
eIF2α (phospho-S51) polyclonal antibody Background :
eIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-eIF2α (S51) polyclonal antibody detects endogenous levels of eIF2αprotein only when phosphorylated at Ser51. Immunogen :
Synthetic phosphopeptide derived from human eIF2α around the phosphorylation site of Serine 51. Conjugate :
Unconjugated Modification :
Phosphorylation
eIF2α (phospho-S51) polyclonal antibody Background :
eIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-eIF2α (S51) polyclonal antibody detects endogenous levels of eIF2αprotein only when phosphorylated at Ser51. Immunogen :
Synthetic phosphopeptide derived from human eIF2α around the phosphorylation site of Serine 51. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-eIF2α (S51) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:The Lung tissue lysate of Mouse(40ug) Lane5:A2780 whole cell lysate(40ug) Lane6:H1792 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-eIF2α (S51) pAb in paraffin-embedded human breast carcinoma tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.