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JNK1/2/3 (phospho-T183) polyclonal antibody BS4763
  • Western blot (WB) analysis of p-JNK1/2/3 (T183) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:K562 whole cell lysate(20ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Kidney tissue lysate of Mouse(20ug)
  • Immunohistochemistry (IHC) analyzes of p-JNK1/2/3 (T183) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Product NameJNK1/2/3 (phospho-T183) polyclonal antibody
Catalog No.BS4763
Swiss-ProtP45983/P45984/P53779
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 46,54 kDa
Alternative NameJNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9;
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylation
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50ul $168
100ul $298
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Product Name :
JNK1/2/3 (phospho-T183) polyclonal antibody
Background :
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-JNK1/2/3 (T183) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183.
Immunogen :
Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183.
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Western blot (WB) analysis of p-JNK1/2/3 (T183) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:K562 whole cell lysate(20ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Kidney tissue lysate of Mouse(20ug)
  • Immunohistochemistry (IHC) analyzes of p-JNK1/2/3 (T183) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Involvement of JNK and P53 Activation in G2/M Cell Cycle Arrest and Apoptosis induced by Titanium Dioxide Nanoparticles in neuron cells PMCID:    Pubmed No.:20863874 Isomalto oligosaccharide sulfate inhibits tumor growth and metastasis of hepatocellular carcinoma in nude mice PMCID:    Pubmed No.:21513518 Benzo(a)pyrene-induced mitochondrial dysfunction and cell death in p53-null Hep3B cells PMCID:    Pubmed No.:21911080 Neurotoxic potential of iron oxide nanoparticles in the rat brain striatum and hippocampus PMCID:    Pubmed No.:22995439 Toosendanin induces apoptosis through suppression of JNK signaling pathway in HL-60 cells PMCID:    Pubmed No.:23111283 RIP140 mediates hyperglycemia-induced glucotoxicity in β-cells via the activation of JNK and ERK1/2 signaling pathways PMCID:    Pubmed No.:23290273 Electromagnetic-pulse-induced activation of p38 MAPK pathway and disruption of blood–retinal barrier PMCID:    Pubmed No.:23570913 miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6. 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PMCID:    Pubmed No.:25322985 Down-regulation of ATF2 in the inhibition of T-2-toxin-induced chondrocyte apoptosis by selenium chondroitin sulfate nanoparticles PMCID:    Pubmed No.:None DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 The osteogenesis-promoting effects of alpha-lipoic acid against glucocorticoid-induced osteoporosis through the NOX4, NF-kappaB, JNK and PI3K/AKT pathways PMCID:    Pubmed No.:28611356 Fenofibrate improves high-fat diet-induced and palmitate-induced endoplasmic reticulum stress and inflammation in skeletal muscle PMCID:    Pubmed No.:27297630 Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway PMCID:    Pubmed No.:28322382 Biochanin A attenuates LPS-induced pro-inflammatory responses and inhibits the activation of the MAPK pathway in BV2 microglial cells PMCID:    Pubmed No.:25483920 Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells PMCID:    Pubmed No.:29285098 Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo PMCID:    Pubmed No.:34648903 Reactive Oxygen Species Play a Biphasic Role in Brain Ischemia PMCID:    Pubmed No.:29420085 Anti-Cancer Effects of Sulfasalazine and Vitamin E Succinate in MDA-MB 231 Triple-Negative Breast Cancer Cells PMCID:    Pubmed No.:31171899 CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway PMCID:    Pubmed No.:34434262 Bone morphogenetic protein 9 serves a protective role in response to ischemic‑reperfusion in the brain by promoting ERK activation PMCID:    Pubmed No.:29257291 Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2 PMCID:    Pubmed No.:31059046 Fuzhuan Brick Tea Boosts Melanogenesis and Prevents Hair Graying through Reduction of Oxidative Stress via NRF2-HO-1 Signaling PMCID:    Pubmed No.:35326249 Regulatory effect of isovitexin on MAPK/NF-κB signal in mice with acute ulcerative colitis PMCID:    Pubmed No.:36394271 Eriocitrin attenuates sepsis-induced acute lung injury in mice by regulating MKP1/MAPK pathway mediated-glycolysis PMCID:    Pubmed No.:36966548 Anti-oxidant and Anti-inflammatory Effects of Ethanol Extract from Polygala sibirica L. var megalopha Fr. on Lipopolysaccharide-Stimulated RAW264.7 Cells PMCID:    Pubmed No.:37434032
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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