ERK 1/2 (phospho-T202) polyclonal antibody 
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Product Name :
ERK 1/2 (phospho-T202) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK 1/2 (T202) polyclonal antibody detects endogenous levels of ERK 1 protein only when phosphorylated at Thr202, and ERK 1 protein only when phosphorylated at Thr185. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202. Conjugate :
Unconjugated Modification :
Phosphorylation
ERK 1/2 (phospho-T202) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK 1/2 (T202) polyclonal antibody detects endogenous levels of ERK 1 protein only when phosphorylated at Thr202, and ERK 1 protein only when phosphorylated at Thr185. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-ERK 1/2 (T202) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with PMA(100nM,15mins) Lane2:H9C2 cell lysate treated with PMA(100nM,15mins) Lane3:PC12 cell lysate treated with PMA(100nM,15mins) -
Western blot (WB) analysis of p-ERK1/2 (T202) pAb at 1:500 dilution Lane1:L02 starved for 16 hours whole cell lysate(40ug) Lane2:L02 starved for 16 hours then treated with LPS(1000ng/ml) for 5 minutes whole cell lysate Lane3:L02 starved for 16 hours then treated with LPS(1000ng/ml) for 15 minutes whole cell lysate Lane4:L02 starved for 16 hours then treated with LPS(1000ng/ml) for 30 minutes whole cell lysate
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.