IkB-α (phospho-Y42) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
IkB-α (phospho-Y42) polyclonal antibody Background :
Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ , and each appear to make essential contributions to IκB phosphorylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-IκB-α (Y42) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Tyr42. Immunogen :
Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Tyrosine 42. Conjugate :
Unconjugated Modification :
Phosphorylation
IkB-α (phospho-Y42) polyclonal antibody Background :
Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ , and each appear to make essential contributions to IκB phosphorylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-IκB-α (Y42) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Tyr42. Immunogen :
Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Tyrosine 42. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-IkB-α (Y42) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:Beas-2B whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-IκB-α (Y42) pAb in paraffin-embedded human breast carcinoma tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.