Product Name :
Smad1 (phospho-S465) polyclonal antibody Background :
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Smad1 (S465) polyclonal antibody detects endogenous levels of Smad1 protein only when phosphorylated at Ser465. Immunogen :
Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of p-Smad1 (S465) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug) Lane3:A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug) Lane4:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane5:A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:The Uterus tissue lysate of Mouse(40
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  Immunohistochemistry (IHC) analyzes of p-Smad1 (S465) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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