Product Name :
p68 RNA Helicase (phospho-Y593) polyclonal antibody Background :
p68 RNA Helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA Helicase activity. p68 RNA Helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA Helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA Helicase increase in serum-induced quiescent cell lines. p68 RNA Helicase may function as a coactivator for estrogen receptor α. Additionally, p68 RNA Helicase associates with transcriptional coactivators CBP and p300. p68 RNA Helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA Helicase and fibrillarin co-localize to nascent nucleoli. p68 RNA Helicase may function as a heterodimer with p72 RNA Helicase. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p68 RNA Helicase (phospho-Y593) polyclonal antibody detects endogenous levels of p68 RNA Helicase protein only when phosphorylated at Tyr593. Immunogen :
Synthetic peptide derived from human p68 RNA Helicase around the phosphorylation site of Y593. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of p68 RNA Helicase (phospho-Y593) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40µg) Lane2:A375 whole cell lysate(40µg) Lane3:U-87MG whole cell lysate(40µg) Lane4:CT-26 whole cell lysate(40µg) Lane5:C6 whole cell lysate(40µg)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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