Eg5 (phospho-T926) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Eg5 (phospho-T926) polyclonal antibody Background :
Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules. Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Eg5 (T926) pAb detects endogenous levels of Eg5 protein only when phosphorylated at Thr926. Immunogen :
Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Threonine 926 Conjugate :
Unconjugated Modification :
Phosphorylation
Eg5 (phospho-T926) polyclonal antibody Background :
Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules. Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Eg5 (T926) pAb detects endogenous levels of Eg5 protein only when phosphorylated at Thr926. Immunogen :
Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Threonine 926 Conjugate :
Unconjugated Modification :
Phosphorylation
-
Western blot (WB) analysis of p-Eg5 (T926) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40µg) Lane2:HepG2 whole cell lysate(40µg) Lane3:CT-26 whole cell lysate(40µg) Lane4:C6 whole cell lysate(40µg) Lane5:LOVO whole cell lysate(40µg) Lane6:MCF-7 whole cell lysate(40µg)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.