PKC-pan (αT497) polyclonal antibody 
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Product Name :
PKC-pan (αT497) polyclonal antibody Background :
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PKC-pan (αT497) polyclonal antibody detects endogenous levels of PKC PKC α, β I, β II, γ, δ, ε, η, ζ and θ isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKCα. Immunogen :
Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497. Conjugate :
Unconjugated Modification :
Unmodification
PKC-pan (αT497) polyclonal antibody Background :
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PKC-pan (αT497) polyclonal antibody detects endogenous levels of PKC PKC α, β I, β II, γ, δ, ε, η, ζ and θ isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKCα. Immunogen :
Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of p-PKC-pan (αT497) pAb at 1:500 dilution Lane1:The Spleen tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:Hela whole cell lysate(20ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-PKC-pan (αT497) pAb in paraffin-embedded human breast carcinoma tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.