TBC1D4 (phospho-T642) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
TBC1D4 (phospho-T642) polyclonal antibody Background :
TBC1 domain family member 4 (TBC1D4), also designated AS160, can be insulin- and/or AKT1-induced. Insulin-stimulated phosphorylation is required for GLUT4 translocation. TBC1D4 may play a role as a GTPase activating protein for proteins in the Rab family. It is expressed primarily in skeletal muscle and heart, as well as spleen, lymph node and leukocytes. Defects in the TBC1D4 gene may cause atopic dermatitis (AD), sometimes referred to as eczema, an atopic chronic skin disease. The skin of affected individuals reacts to irritants or allergens and becomes red, flaky and itchy. The skin is also more vulnerable to inflammations, and symptoms can grow or disappear over time. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-TBC1D4 (T642) polyclonal antibody detects endogenous levels of TBC1D4 only when phosphorylated at Thr642. Immunogen :
Synthetic phosphopeptide derived from human TBC1D4 around the phosphorylation site of Threonine 642. Conjugate :
Unconjugated Modification :
Phosphorylation
TBC1D4 (phospho-T642) polyclonal antibody Background :
TBC1 domain family member 4 (TBC1D4), also designated AS160, can be insulin- and/or AKT1-induced. Insulin-stimulated phosphorylation is required for GLUT4 translocation. TBC1D4 may play a role as a GTPase activating protein for proteins in the Rab family. It is expressed primarily in skeletal muscle and heart, as well as spleen, lymph node and leukocytes. Defects in the TBC1D4 gene may cause atopic dermatitis (AD), sometimes referred to as eczema, an atopic chronic skin disease. The skin of affected individuals reacts to irritants or allergens and becomes red, flaky and itchy. The skin is also more vulnerable to inflammations, and symptoms can grow or disappear over time. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-TBC1D4 (T642) polyclonal antibody detects endogenous levels of TBC1D4 only when phosphorylated at Thr642. Immunogen :
Synthetic phosphopeptide derived from human TBC1D4 around the phosphorylation site of Threonine 642. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-TBC1D4 (T642) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:Panc1 whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-TBC1D4 (T642) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Human Mesenchymal Stem Cell Derived Exosomes Alleviate Type 2 Diabetes Mellitus by Reversing Peripheral Insulin Resistance and Relieving β-Cell Destruction
PMCID: Pubmed No.:30052036
ErbB1-dependent signalling and vesicular trafficking in primary afferent nociceptors associated with hypersensitivity in neuropathic pain
PMCID: Pubmed No.:32531343
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.