Insulin Receptor (phospho-Y1355) polyclonal antibody 
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Product Name :
Insulin Receptor (phospho-Y1355) polyclonal antibody Background :
The insulin receptor (IR) is a heterodimeric protein complex that has an intracellular β subunit and an extracellular α subunit, which is disulfide- linked to a transmembrane segment. The insulin ligand binds to the IR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Insulin Receptor (Y1355) polyclonal antibody detects endogenous levels of Insulin Receptor protein only when phosphorylated at Tyr1355. Immunogen :
Synthetic phosphopeptide derived from human Insulin Receptor around the phosphorylation site of Tyrosine 1355. Conjugate :
Unconjugated Modification :
Phosphorylation
Insulin Receptor (phospho-Y1355) polyclonal antibody Background :
The insulin receptor (IR) is a heterodimeric protein complex that has an intracellular β subunit and an extracellular α subunit, which is disulfide- linked to a transmembrane segment. The insulin ligand binds to the IR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Insulin Receptor (Y1355) polyclonal antibody detects endogenous levels of Insulin Receptor protein only when phosphorylated at Tyr1355. Immunogen :
Synthetic phosphopeptide derived from human Insulin Receptor around the phosphorylation site of Tyrosine 1355. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-Insulin Receptor (Y1355) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-Insulin Receptor (Y1355) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain
PMCID: Pubmed No.:26779933
Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain
PMCID: Pubmed No.:26779933
Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain
PMCID: Pubmed No.:26779933
Methamphetamine exposure upregulates the amyloid precursor protein and hyperphosphorylated tau expression: The roles of insulin signaling in SH-SY5Y cell line
PMCID: Pubmed No.:31270305
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.