Tau (phospho-T231) polyclonal antibody 
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MSDS
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Product Name :
Tau (phospho-T231) polyclonal antibody Background :
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Tau (T231) polyclonal antibody detects endogenous levels of Tau only when phosphorylated at Thr548, isoforms Tau-3 and isoforms Tau-4 phosphorylated at Thr231, and other isoforms phosphorylated at the corresponding Site. Immunogen :
Synthetic phosphopeptide derived from human isoforms Tau-4 around the phosphorylation site of Threonine 231. Conjugate :
Unconjugated Modification :
Phosphorylation
Tau (phospho-T231) polyclonal antibody Background :
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Tau (T231) polyclonal antibody detects endogenous levels of Tau only when phosphorylated at Thr548, isoforms Tau-3 and isoforms Tau-4 phosphorylated at Thr231, and other isoforms phosphorylated at the corresponding Site. Immunogen :
Synthetic phosphopeptide derived from human isoforms Tau-4 around the phosphorylation site of Threonine 231. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of Tau (phospho-T231) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:Hela treated with EGF(0.1ng/ml, 30min) whole cell lysate(40μg) Lane3:C6 whole cell lysate(40μg) Lane4:C6 treated with EGF(0.1ng/ml, 30min) whole cell lysate(40μg)
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
Self-assembling nanofibers alter the processing of amyloid precursor protein in a transgenic mouse model of Alzheimer's disease
PMCID: Pubmed No.:25005263
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
The Involvement of NR2B and tau Protein in MG132-Induced CREB Dephosphorylation
PMCID: Pubmed No.:28424976
Fecal microbiota transplantation alleviated Alzheimer’s disease-like pathogenesis in APP/PS1 transgenic mice
PMCID: Pubmed No.:31383855
Chronic noise exposure exacerbates AD-like neuropathology in SAMP8 mice in relation to Wnt signaling in the PFC and hippocampus
PMCID: Pubmed No.:30279527
Ferroptosis promotes microtubule-associated protein tau aggregation via GSK-3β activation and proteasome inhibition
PMCID: Pubmed No.:34997541
Effect of Probiotic Fungi against Cognitive Impairment in Mice via Regulation of the Fungal Microbiota–Gut–Brain Axis
PMCID: Pubmed No.:35833673
Microbiota-derived metabolite Indoles induced aryl hydrocarbon receptor activation and inhibited neuroinflammation in APP/PS1 mice
PMCID: Pubmed No.:35961580
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.