PYK2 (phospho-Y580) polyclonal antibody 
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MSDS
SHIP
Product Name :
PYK2 (phospho-Y580) polyclonal antibody Background :
Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PYK2 (Y580) polyclonal antibody detects endogenous levels of PYK2 protein only when phosphorylated at Tyr580. Immunogen :
Synthetic phosphopeptide derived from human PYK2 around the phosphorylation site of Tyrosine 580. Conjugate :
Unconjugated Modification :
Phosphorylation
PYK2 (phospho-Y580) polyclonal antibody Background :
Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PYK2 (Y580) polyclonal antibody detects endogenous levels of PYK2 protein only when phosphorylated at Tyr580. Immunogen :
Synthetic phosphopeptide derived from human PYK2 around the phosphorylation site of Tyrosine 580. Conjugate :
Unconjugated Modification :
Phosphorylation
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Immunohistochemistry (IHC) analyzes of p-PYK2 (Y580) pAb in paraffin-embedded human colon cancer tissue.
Modeled microgravity causes changes in the cytoskeleton and focal adhesions, and decreases in migration in malignant human MCF-7 cells
PMCID: Pubmed No.:19730978
High Expression of Proline-Rich Tyrosine Kinase2 is Associated with Poor Survival of Hepatocellular Carcinoma via Regulating Phosphatidylinositol 3-Kinase/AKT Pathway
PMCID: Pubmed No.:22618716
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.