Product Name :
Rpb1 (phospho-S1619) polyclonal antibody Background :
RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Rpb1 (S1619) polyclonal antibody detects endogenous levels of Rpb1 protein only when phosphorylated at Ser1619. Immunogen :
Synthetic phosphopeptide derived from human Rpb1 around the phosphorylation site of Serine 1619. Conjugate :
Unconjugated Modification :
Phosphorylation

• 
  Western blot (WB) analysis of p-Rpb1 (S1619) pAb at 1:2000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:3T3-L1 whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of p-Rpb1 (S1619) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---