Product Name :
SMC 1 (phospho-S957) polyclonal antibody Background :
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein, and Bamacan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed as a 150 kDa protein in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a 146 kDa nuclear protein in the colon, but can also occur as a secreted proteoglycan (called Bamacan) expressed in testis and brain. Bamacan contains several glycosylation sites and is thought to play a role in basement membrane physiology. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-SMC1 (S957) polyclonal antibody detects endogenous levels of SMC1protein only when phosphorylated at Ser957 Immunogen :
Synthetic phosphopeptide derived from human SMC 1 around the phosphorylation site of Serine 957. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of p-SMC 1 (S957) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:SGC7901 treated with UV for 5 minutes then repair for 1 hours whole cell lysate(40ug) Lane3:SGC7901 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug) Lane4:SGC7901 treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analysis of p-SMC1 (S957) pAb in paraffin-embedded human lung carcinoma tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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