Product Name :
NFkB-p100 (phospho-S865) polyclonal antibody Background :
The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-NFkB-p100 (S865) polyclonal antibody pAb detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser865. Immunogen :
Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 865. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of NFkB-p100 (phospho-S865) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate(40ug) Lane2:NIH-3T3(PMA, 60ng/ml, 5min)whole cell lysate(40ug) Lane3:NIH-3T3(PMA, 60ng/ml, 15min)whole cell lysate(40ug) Lane4:NIH-3T3(PMA, 60ng/ml, 30min)whole cell lysate(40ug) Lane5:NIH-3T3(EGF, 0.1ng/ml, 30min)whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of p-NFkB-p100 (S865) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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