CrkL (phospho-Y207) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CrkL (phospho-Y207) polyclonal antibody Background :
Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207. Immunogen :
Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207. Conjugate :
Unconjugated Modification :
Phosphorylation
CrkL (phospho-Y207) polyclonal antibody Background :
Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207. Immunogen :
Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-CrkL (Y207) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-Crk-L (Y207) pAb in paraffin-embedded human colon cancer tissue.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.