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TCEAL1 (E123) polyclonal antibody BS3956
  • Western blot (WB) analysis of TCEAL1 (E123) pAb at 1:500 dilution Lane1:A2780 whole cell lysate(20ug) Lane2:PC3 whole cell lysate(20ug) Lane3:The Ovary tissue lysate of Rat(40ug) Lane4:3T3-L1 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of TCEAL1 (E123) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Product NameTCEAL1 (E123) polyclonal antibody
Catalog No.BS3956
Swiss-ProtQ15170
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 18 kDa
Alternative NameTCEA-like protein 1; TCEAL1; pp21; SIIR;
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
TCEAL1 (E123) polyclonal antibody
Background :
TCEAL1 (transcription elongation factor A (SII) protein 3), also known as p21, SIIR or pp21, is a member of the TFS-II family. Transcription elongation factors of the TFS-II family are responsible for releasing RNA polymerase II (Pol II) from transcriptional arrest. DNA arresting sites can result in locked ternary complexes if elongating RNA polymerases are trapped. Transcription elongation factors function to activate the intrinsic RNA cleavage activity of RNA polymerases. This allows the RNA polymerase to cleave the nascent transcript, thereby forming a new 3’-terminus to resume elongation. TCEAL1 is a 157 amino acid nuclear protein that is ubiquitously expressed. TCEAL1 may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
TCEAL1 (E123) polyclonal antibody detects endogenous levels of TCEAL1 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 90-143 of Human TCEAL1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of TCEAL1 (E123) pAb at 1:500 dilution Lane1:A2780 whole cell lysate(20ug) Lane2:PC3 whole cell lysate(20ug) Lane3:The Ovary tissue lysate of Rat(40ug) Lane4:3T3-L1 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of TCEAL1 (E123) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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