Product Name :
Rab 6A (K144) polyclonal antibody Background :
Rab proteins are also an integral part of endocytic pathways. Rab 6A is a ubiquitously expressed member of the Rab family of proteins and localizes to the Golgi membrane where it regulates retrograde transport from the late endosomes via the Golgi to endoplasmic reticulum (ER) pathway. Three isoforms exist due to alternative splicing events, namely the ubiquitously expressed isoforms Rab 6A' and Rab 6A, and the brain-specific isoform Rab 6B. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Rab 6A (K144) polyclonal antibody detects endogenous levels of Rab 6A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 120-170 of Human Rab 6A. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Rab 6A (K144) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:The Testis tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug)
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  Immunohistochemistry (IHC) analyzes of Rab 6A (K144) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and membranous staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of Rab 6A (K144) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and membranous staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of Rab 6A (K144) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and membranous staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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