Product Name :
GSTT1 (R31) polyclonal antibody Background :
The GST superfamily is made up of several subfamilies. Glutathione S-transferase P (GSTP1) belongs to the Pi subfamily and is involved in the conjugation of reduced glutathione to a variety of endogenous levels of and exogenous hydrophobic electrophiles. Glutathione S-transferase Mu 1 (GSTM1) is a cytoplasmic liver protein belonging to the Mu family and has the same basic functions as GSTP1-1. Glutathione S-transferase theta 1 (GSTT1), a cytoplasmic homodimer belonging to the Theta family, is expressed in erythrocytes. It is active in the reduced glutathione conjugation and also displays glutathione peroxidase activity with cumene hydroperoxide. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GSTT1 (R31) polyclonal antibody detects endogenous levels of GSTT1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1-50 of Human GSTT1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of GSTT1 (R31) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:HCC827 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:AML-12 whole cell lysate(40ug) Lane7:HepG2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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