Product Name :
Connexin 37 (L116) polyclonal antibody Background :
The Connexin 37/GJA4 gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Connexin 37 (L116) polyclonal antibody detects endogenous levels of Connexin 37 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Connexin 37. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Connexin 37 (L116) pAb at 1:1000 dilution Lane1:SK-OVCAR3 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(20ug) Lane3:HEK293T whole cell lysate(20ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug) Lane6:PC12 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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