CLIC3 (F56) polyclonal antibody 
Datasheet
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Product Name :
CLIC3 (F56) polyclonal antibody Background :
CLIC3 (chloride intracellular channel protein 3) is a member of the highly conserved family of chloride ion channels (CLICs) that function in both soluble and integral membrane forms. Chloride channels regulate cellular traffic of chloride ions, a critical component of all living cells. They are involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport. CLIC3 is found in a variety of tissues but is highly expressed in placenta, brain and heart. CLIC3 predominantly localizes to the nucleus and stimulates chloride ion channel activity when expressed in cells. In addition, CLIC3 interacts with ERK 7 and may play a role in the regulation of cell proliferation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CLIC3 (F56) polyclonal antibody detects endogenous levels of CLIC3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 31-80 of Human CLIC3. Conjugate :
Unconjugated Modification :
Unmodification
CLIC3 (F56) polyclonal antibody Background :
CLIC3 (chloride intracellular channel protein 3) is a member of the highly conserved family of chloride ion channels (CLICs) that function in both soluble and integral membrane forms. Chloride channels regulate cellular traffic of chloride ions, a critical component of all living cells. They are involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport. CLIC3 is found in a variety of tissues but is highly expressed in placenta, brain and heart. CLIC3 predominantly localizes to the nucleus and stimulates chloride ion channel activity when expressed in cells. In addition, CLIC3 interacts with ERK 7 and may play a role in the regulation of cell proliferation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CLIC3 (F56) polyclonal antibody detects endogenous levels of CLIC3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 31-80 of Human CLIC3. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CLIC3 (F56) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:HCC827 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:H1792 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.